Circulating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac disease
K. S. Amr1, F. S. Bayoumi2, E. Eissa emanessa82@yahoo.com2, M. Abu-Zekry3Show more: Authors information and Publication history1Medical molecular genetics Department, National Research Centre, Cairo, Egypt
2Immunogenetics Department, National Research Centre, Cairo, Egypt
3Abu El Reesh Children's Hospital, Cairo University, Cairo, Egypt
Publication History: Published online: 11 April 2019
Accepted: 04 February 2018
Received: 23 September 2018
Doihttps://doi.org/10.23822/EurAnnACI.1764-1489.90
Abstract
Celiac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients.
Key words
celiac disease, microRNA, gluten free diet, biomarkers, auto-antibodies.