1Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
2Systems Biology of Diseases Research Unit, Faculty of Science, Mahidol University, Bangkok, Thailand
3Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand
4Institute of Molecular Biosciences, Mahidol University, Nakhonpathom, Thailand
5Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
6Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
7Division of Rhinology and Allergy, Department of Otorhinolaryngology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
8Department of Agriculture, Faculty of Agriculture, Niigata University, Niigata, Japan
9Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, Thailand
Publication History: Published online: 12 April 2019
Accepted: 25 March 2019
Received: 02 October 2018
Group 1 grass pollen allergens, or beta-expansins, are the most important major allergens from tropical/subtropical grasses. This study aimed to investigate the sequence similarity, and immunoreactivity of group 1 allergens from Para grass (Urochloa mutica). Three isoforms (Uro m 1.01, Uro m 1.02, and Uro m 1.03) were cloned from cDNA of Para grass pollen. The acidic-neutral isoforms rUro m 1.01 and rUro m 1.02 could effectively inhibited beta-expansins in pollen extract of Bermuda and Johnson grasses, suggesting that these isoforms could be major cross-reacting allergens among these grasses. In contrast, the basic isoform rUro m 1.03 had limited IgE reactivity. Thus, group 1 allergens both acidic-neutral and basic isoforms could have markedly different IgE reactivity.
Para grass pollen, beta-expansins, isoallergens, Uro m 1, recombinant group-1 allergen