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Table of Contents »

Banana lectin (BanLec) induces non-specific activation of basophils and mast cells in atopic subjects


N. Krithika1, S.N. Pramod1, P.A. Mahesh2, Y.P. Venkatesh1 venkatyp@yahoo.com

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Doi
https://doi.org/10.23822/EurAnnACI.1764-1489.64

Summary
Dietary lectins play a major role in the activation of mast cells / basophils by bridging cell surface IgE glycans to release histamine and other mediators. In the present study, the effect of mannose / glucose-specific banana lectin (BanLec) on the activation of mast cells / basophils from non-atopic and atopic subjects has been investigated. BanLec was purified from banana pulp in a yield of 7 mg/kg. Leukocytes isolated from heparinized blood of non-atopic / atopic subjects were used for quantitation of the released histamine. Approximately 28.2% of the atopics (n = 117) was positive by skin prick test (SPT) to purified BanLec (100 µg/mL concentration), and all the non-atopics (n = 20) were negative. Maximal release of histamine was seen at 2 µg of BanLec. In percent histamine release, an increase of 35-40% is observed in case of atopics (n = 7) compared to non-atopics (n = 5), and the histamine release from atopic and non-atopic subjects correlates fairly well with the total serum IgE levels (R2 = 0.817). BanLec also induces release of histamine (26.7%) from mast cells present in rat peritoneal exudate cells. BanLec can significantly activate and degranulate mast cells and basophils by cross-linking the trimannosidic core mannose of IgE glycans in atopic population as compared to non-atopic population; the activation is marginal in the case of non-atopics.

Key words
atopics; banana lectin; histamine release; serum IgE; skin prick test


Cite this article as:
Krithika K, Pramod S.N, P.A. Mahesh, Venkatesh Y.P. Banana lectin (BanLec) induces non-specific activation of basophils and mast cells in atopic subjects. Eur Ann Allergy Clin Immunol. 2018;50(6):243-253. doi:10.23822/EurAnnACI.1764-1489.64

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