European Annals of Allergy and Clinical Immunology THE OFFICIAL JOURNAL OF AAITO THE OFFICIAL JOURNAL OF SPAIC HOME PAGE

EDITORS IN CHIEF
L. Cecchi (Firenze - Italy)

P. Carreiro-Martins (Lisbon - Portugal)

 

HONORARY EDITOR
A. Sabbah (Angers - France)

 

ASSOCIATE EDITORS
R. Rodrigues Alves (Lisbon - Portugal)
A. Tedeschi (Milano - Italy)

 

EDITORIAL BOARD
M. Morais-Almeida (Lisbon - Portugal)
R. Asero (Milan - Italy)
M.B. Bilò (Ancona - Italy)
F. Bonifazi (Ancona - Italy)
L.M. Borrego (Lisbon - Portugal)

K. Brockow (München - Germany)
A.À. Cruz (Salvador - Brasil)
L. Delgado (Oporto - Portugal)
P. Demoly (Montpellier - France)
G. D'Amato (Napoli - Italy)
M. Drouet (Angers - France)
M. Fernandez-Rivas (Madrid - Spain)
A. Fiocchi (Milano - Italy)

J. Fonseca (Oporto - Portugal)
D. Macchia (Firenze - Italy)
F. Mastrandrea (Taranto - Italy)
M. Maurer (Berlin - Germany)
G. Moscato (Pavia - Italy)
A. Musarra (Reggio Calabria - Italy)
C. Nunes (Portimao - Portugal)
M. Olivieri (Verona - Italy)
P. Parronchi (Firenze - Italy)
G. Passalacqua (Genova - Italy)
G. Pauli (Strasbourg - France)
E. Pedro (Lisbon - Portugal)
A. Perino (Torino - Italy)
L.K. Poulsen (Copenaghen - Denmark)
O. Quercia (Faenza - Italy)
A. Romano (Roma - Italy)
E. Scala (Roma - Italy)
D. Solé (Sao Paulo - Brasil)
A. Todo Bom (Coimbra - Portugal)
S. Voltolini (Genova - Italy)

 

SCIENTIFIC COMMITTEE
L. Antonicelli (Italy)
A. Bener (Turkey)
H. Bazin (Belgium)
J. Bellanti (USA)
C. Geller-Bernstein (Israel)
M. Cugno (Italy)
B. David (France)
S. Durham (UK)
G.P. Girolomoni (Italy)
R. Jarish (Austria)
S.G.O. Johansson (Sweden)
F. Levi-Shaffer (Israel)
H. Lowenstein (Denmark)
J.L. Malo (Canada)
A.G. Palma-Carlos (Portugal)
G. Scadding (UK)
G. Scadding (UK)
E. Stevens (Belgium)
R. van Ree (Netherlands)

 

FOUNDER AND CORRESPONDING MEMBER
G.M. Halpern (USA)


*************************

Editors in Chief
Lorenzo Cecchi
Pedro Carreiro-Martins

Chief Business & Content Officer
Ludovico Baldessin

Publishing Editor

Chiara Scelsi

c.scelsi@lswr.it
Ph. 0039 (0)2-88184.257


Production Manager

Walter Castiglione
w.castiglione@lswr.it
Ph. 0039 (0)2-88184.222

Sales
Stefano Busconi
dircom@lswr.it
Ph. 0039 (0)2-88184.404

Printing
Mccgraphics
Pol. Ind. Txirrita Maleo Pab 11
20100 Errenteria (Gipuzkoa), Spain

EDRA SpA
Via G. Spadolini, 7
20141 Milano - Italy
Ph. 0039 (0)2-88184.1
Fax 0039 (0)2-88184.301 
www.edizioniedra.it

Table of Contents »

Latex sensitization in patients with myelomeningocele: contribution of microarray technique


A.B. de Sá, L.C. Oliveira, R. Camilo, F.F. Pierotti, D. Solé dirceu.sole@unifesp.br

Show more: Authors information and Publication history

Doi
https://doi.org/10.23822/EurAnnACI.1764-1489.52

Summary
Background
. Microarray technique employing molecular allergens is pointed out  as a new method to evaluate allergic patients. Objective. To evaluate if mi-croarray technique (ImmunoCAP-ISAC®; I-ISAC®) is similar to fluorescence en-zyme immunoassay (FEIA; ImmunoCAP®) in the diagnosis of latex allergy (specific IgE to latex plus symptoms) and latex sensitization (only antibody) in pediatric (9-mo to 14-yrs) patients with myelomeningocele  undergone to surgical repair. Methods. Patients underwent skin prick testing (SPT) to latex and food (prick to prick), and dosage of serum specific IgE to latex and recombinant latex allergens (rHev b 1, rHev b 3, rHev b 5, rHev b 6.01, rHev b 6.02, rHev b 8, rHev b 9, and rHev b 11) by ImmunoCAP® and I-ISAC®. Results. The comparison between the two  methods showed high level of concordance considering positive and negative results. A statistically significant correlation for rHev b 3 and rHev b 6.01 for the allergic patients, and for rHev b5 and rHev b6.01 for those sensitized to latex, was  observed. I-ISAC® is limited to 5 recombinant latex allergens (rHev b 1, 3, 5, 6.01 and 8). Despite the presence of antibodies against pollens, LTP and profilins (I-ISAC®) in some patients, none of them reported symptoms related with intake of fruits and/or vegetables. Conclusion. Both methods are effective in assisting the diagnosis of latex allergy, but differ in the assessment of sensitized pediatric patients with myelomeningocele. The assessment of latex sensitized pa-tients is more complete using the 8 recombinant latex fractions available for Im-munoCAP®, instead of I-ISAC®.

Key words
latex; myelomeningocele; recombinant allergens; latex-fruit syndrome; allergy


Cite this article as:
de Sá AB, Oliveira LC, Camilo R, Pierotti FF, Solé D. Latex sensitization in patients with myelomeningocele: contribution of microarray technique. Eur Ann Allergy Clin Immunol. 2018;50(3):135-138. doi:10.23822/EurAnnACI.1764-1489.52

FULL TEXT